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gl261 nc  (Boster Bio)


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    Boster Bio gl261 nc
    Characterization of murine glioma cell lines <t>(GL261,</t> CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .
    Gl261 Nc, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gl261 nc/product/Boster Bio
    Average 93 stars, based on 36 article reviews
    gl261 nc - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses"

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    Journal: Cancers

    doi: 10.3390/cancers14092303

    Characterization of murine glioma cell lines (GL261, CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .
    Figure Legend Snippet: Characterization of murine glioma cell lines (GL261, CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .

    Techniques Used: shRNA, Knockdown, Expressing, Western Blot, Activity Assay, Control

    Metabolic energy generation of NC and LDH-A KD murine glioma cell lines . Glycolytic proton efflux rate (glycoPER): basal ( A ) and compensatory glycolysis ( B ) assessed by Seahorse XF analyzer (30,000 of NC and LDH-A KD GL261, CT2A, ALTC1S1 cells were seeded 4 h before the experiments). Results were normalized per 10,000 cells (n = 6, mean ± SEM); Rot/AA, rotenone + antimycin A; 2-DG, 2-Deoxy-D-glucose). Energy map of six tested cell lines charting mitochondrial ATP (mito ATP) versus glycolysis-generated ATP (glycol ATP) production; mean ± SD ( C ). Representative profiles of real-time glycoPER for GL261 ( D ), CT2A ( E ) and ALTS1C1 ( F ) cell lines, comparing NC and LDH-A KD. Representative profiles of a real-time OCR profile for GL261 ( G ), CT2A ( H ) and ALTS1C1 ( I ) cell lines, comparing NC and LDH-A KD. Values are mean, ±SEM; n = 5 (GL261); 4 (CT2A); 7 (ALTS1C1).
    Figure Legend Snippet: Metabolic energy generation of NC and LDH-A KD murine glioma cell lines . Glycolytic proton efflux rate (glycoPER): basal ( A ) and compensatory glycolysis ( B ) assessed by Seahorse XF analyzer (30,000 of NC and LDH-A KD GL261, CT2A, ALTC1S1 cells were seeded 4 h before the experiments). Results were normalized per 10,000 cells (n = 6, mean ± SEM); Rot/AA, rotenone + antimycin A; 2-DG, 2-Deoxy-D-glucose). Energy map of six tested cell lines charting mitochondrial ATP (mito ATP) versus glycolysis-generated ATP (glycol ATP) production; mean ± SD ( C ). Representative profiles of real-time glycoPER for GL261 ( D ), CT2A ( E ) and ALTS1C1 ( F ) cell lines, comparing NC and LDH-A KD. Representative profiles of a real-time OCR profile for GL261 ( G ), CT2A ( H ) and ALTS1C1 ( I ) cell lines, comparing NC and LDH-A KD. Values are mean, ±SEM; n = 5 (GL261); 4 (CT2A); 7 (ALTS1C1).

    Techniques Used: Generated

    Overexpression of genes involved in oxidative phosphorylation in GL261 LDH-A KD cells. Transcripts per million (TPM) expression values were plotted in individual cell lines for genes directly involved in lactate metabolism and export (LDH-A, LDH-B, and SLC16A3) ( A ). Gene Set Enrichment Analysis (GSEA) for a single pathway (GO_OXIDATIVE_ PHOSPHORYLATION) is shown for each GBM cell line. The analysis shows the enrichment of this pathway in LDH-A-depleted vs. control cells ( B ). TPM values for enzymes involved in oxidative phosphorylation ( C ). The Z-transformed scores of individual genes within the oxidative phosphorylation (GO) pathway were plotted across each cell line (GL261, CT2A and ALTS1C1). The experiment was performed in triplicate, with rows representing each sample and columns representing individual genes ( D ). Expression (TPM) of pyruvate dehydrogenase alpha 1 (PDHA1) and aconitase 1 (ACO1) was plotted ( E ). PDHA1 is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA; ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle. Significant differences are indicated by: * p < 0.05, ** p < 0.01, and *** p < 0.001. provides the semi-raw data from the RNASeq analysis that was used to generate the figures.
    Figure Legend Snippet: Overexpression of genes involved in oxidative phosphorylation in GL261 LDH-A KD cells. Transcripts per million (TPM) expression values were plotted in individual cell lines for genes directly involved in lactate metabolism and export (LDH-A, LDH-B, and SLC16A3) ( A ). Gene Set Enrichment Analysis (GSEA) for a single pathway (GO_OXIDATIVE_ PHOSPHORYLATION) is shown for each GBM cell line. The analysis shows the enrichment of this pathway in LDH-A-depleted vs. control cells ( B ). TPM values for enzymes involved in oxidative phosphorylation ( C ). The Z-transformed scores of individual genes within the oxidative phosphorylation (GO) pathway were plotted across each cell line (GL261, CT2A and ALTS1C1). The experiment was performed in triplicate, with rows representing each sample and columns representing individual genes ( D ). Expression (TPM) of pyruvate dehydrogenase alpha 1 (PDHA1) and aconitase 1 (ACO1) was plotted ( E ). PDHA1 is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA; ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle. Significant differences are indicated by: * p < 0.05, ** p < 0.01, and *** p < 0.001. provides the semi-raw data from the RNASeq analysis that was used to generate the figures.

    Techniques Used: Over Expression, Phospho-proteomics, Expressing, Control, Transformation Assay

    The effect of LDH-A knock-down on cells in vitro and s.c. tumor growth, in vivo in immune-competent mice . Growth profiles and doubling times of GL261, ALTS1C1 and CT2A cells in vitro (Panels A – D ) (mean ± SEM) and tumors in C57BL/6 mice (Panels E – H ) with and without LDH-A shRNA knock-down (mean, ± SD). Note that the doubling times for GL261 NC tumors (Panel E) were estimated after the initial delay in tumor growth (0~40 days).
    Figure Legend Snippet: The effect of LDH-A knock-down on cells in vitro and s.c. tumor growth, in vivo in immune-competent mice . Growth profiles and doubling times of GL261, ALTS1C1 and CT2A cells in vitro (Panels A – D ) (mean ± SEM) and tumors in C57BL/6 mice (Panels E – H ) with and without LDH-A shRNA knock-down (mean, ± SD). Note that the doubling times for GL261 NC tumors (Panel E) were estimated after the initial delay in tumor growth (0~40 days).

    Techniques Used: Knockdown, In Vitro, In Vivo, shRNA

    The effect of LDH-A knock-down on s.c. tumor growth in nude mice . Growth profiles of GL261, ALTS1C1 and CT2A tumors (Panels A – C ), and estimated tumor doubling times (Panel D ). Mean, ± SD. Comparison between tumor doubling times in nude mice and C57BL/6 mice (Panel E ).
    Figure Legend Snippet: The effect of LDH-A knock-down on s.c. tumor growth in nude mice . Growth profiles of GL261, ALTS1C1 and CT2A tumors (Panels A – C ), and estimated tumor doubling times (Panel D ). Mean, ± SD. Comparison between tumor doubling times in nude mice and C57BL/6 mice (Panel E ).

    Techniques Used: Knockdown, Comparison

    Native-polyacrylamide gel electrophoresis LDH zymograms for ex vivo tissue and s.c. GL261, CT2A and ALTS1C2 tumors. Electrophoretic patterns in the heart and skeletal muscle as well as s.c. tumors from NC and LDH-A KD GL261, CT2A and ALTS1C1 tumors (Panel A ), corresponding LDH isoform profiles (Panel B ); n = five independent studies.
    Figure Legend Snippet: Native-polyacrylamide gel electrophoresis LDH zymograms for ex vivo tissue and s.c. GL261, CT2A and ALTS1C2 tumors. Electrophoretic patterns in the heart and skeletal muscle as well as s.c. tumors from NC and LDH-A KD GL261, CT2A and ALTS1C1 tumors (Panel A ), corresponding LDH isoform profiles (Panel B ); n = five independent studies.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Ex Vivo

    H&E and IHC staining for LDH-A and LDH-B protein expression in s.c. GL261 and CT2A tumors—both LDH-A KD and NC controls . H&E staining for GL261 NC and LDH-A KD tumors ( Aa ); LDH-A staining ( Ab ) and LDH-B staining ( Ac ). GL261 NC and LDH-A KD tumors were grown in immunocompromised nude mice. Quantification of percentage LDH-A tumor expression ( B ) and percentage LDH-B tumor expression ( C ); ±SEM. A similar presentation is shown for CT2A tumors, grown in immune-competent C57BL/6 mice (Panels D – F ).
    Figure Legend Snippet: H&E and IHC staining for LDH-A and LDH-B protein expression in s.c. GL261 and CT2A tumors—both LDH-A KD and NC controls . H&E staining for GL261 NC and LDH-A KD tumors ( Aa ); LDH-A staining ( Ab ) and LDH-B staining ( Ac ). GL261 NC and LDH-A KD tumors were grown in immunocompromised nude mice. Quantification of percentage LDH-A tumor expression ( B ) and percentage LDH-B tumor expression ( C ); ±SEM. A similar presentation is shown for CT2A tumors, grown in immune-competent C57BL/6 mice (Panels D – F ).

    Techniques Used: Immunohistochemistry, Expressing, Staining



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    gl261 nc  (Boster Bio)
    93
    Boster Bio gl261 nc
    Characterization of murine glioma cell lines <t>(GL261,</t> CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .
    Gl261 Nc, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gl261 nc/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    gl261 nc - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of murine glioma cell lines (GL261, CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: Characterization of murine glioma cell lines (GL261, CT2A and ALTS1C1) following LDH-A shRNA knock-down . LDH-A and LDH-B mRNA levels by ddPCR ( A , B ); protein expression on Western blot analyses ( C – E ); and LDH enzyme activity ( F ) in control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). LDH-B/LDH-A mRNA expression ratios ( G ) and LDH-B/LDH-A Western blot ratios ( H ) for control NC and LDH-A KD cell lines (GL261, CT2A, ALTS1C1). n = 3, ±SEM. The native Western blot for Panel E is shown in the .

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: shRNA, Knockdown, Expressing, Western Blot, Activity Assay, Control

    Metabolic energy generation of NC and LDH-A KD murine glioma cell lines . Glycolytic proton efflux rate (glycoPER): basal ( A ) and compensatory glycolysis ( B ) assessed by Seahorse XF analyzer (30,000 of NC and LDH-A KD GL261, CT2A, ALTC1S1 cells were seeded 4 h before the experiments). Results were normalized per 10,000 cells (n = 6, mean ± SEM); Rot/AA, rotenone + antimycin A; 2-DG, 2-Deoxy-D-glucose). Energy map of six tested cell lines charting mitochondrial ATP (mito ATP) versus glycolysis-generated ATP (glycol ATP) production; mean ± SD ( C ). Representative profiles of real-time glycoPER for GL261 ( D ), CT2A ( E ) and ALTS1C1 ( F ) cell lines, comparing NC and LDH-A KD. Representative profiles of a real-time OCR profile for GL261 ( G ), CT2A ( H ) and ALTS1C1 ( I ) cell lines, comparing NC and LDH-A KD. Values are mean, ±SEM; n = 5 (GL261); 4 (CT2A); 7 (ALTS1C1).

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: Metabolic energy generation of NC and LDH-A KD murine glioma cell lines . Glycolytic proton efflux rate (glycoPER): basal ( A ) and compensatory glycolysis ( B ) assessed by Seahorse XF analyzer (30,000 of NC and LDH-A KD GL261, CT2A, ALTC1S1 cells were seeded 4 h before the experiments). Results were normalized per 10,000 cells (n = 6, mean ± SEM); Rot/AA, rotenone + antimycin A; 2-DG, 2-Deoxy-D-glucose). Energy map of six tested cell lines charting mitochondrial ATP (mito ATP) versus glycolysis-generated ATP (glycol ATP) production; mean ± SD ( C ). Representative profiles of real-time glycoPER for GL261 ( D ), CT2A ( E ) and ALTS1C1 ( F ) cell lines, comparing NC and LDH-A KD. Representative profiles of a real-time OCR profile for GL261 ( G ), CT2A ( H ) and ALTS1C1 ( I ) cell lines, comparing NC and LDH-A KD. Values are mean, ±SEM; n = 5 (GL261); 4 (CT2A); 7 (ALTS1C1).

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Generated

    Overexpression of genes involved in oxidative phosphorylation in GL261 LDH-A KD cells. Transcripts per million (TPM) expression values were plotted in individual cell lines for genes directly involved in lactate metabolism and export (LDH-A, LDH-B, and SLC16A3) ( A ). Gene Set Enrichment Analysis (GSEA) for a single pathway (GO_OXIDATIVE_ PHOSPHORYLATION) is shown for each GBM cell line. The analysis shows the enrichment of this pathway in LDH-A-depleted vs. control cells ( B ). TPM values for enzymes involved in oxidative phosphorylation ( C ). The Z-transformed scores of individual genes within the oxidative phosphorylation (GO) pathway were plotted across each cell line (GL261, CT2A and ALTS1C1). The experiment was performed in triplicate, with rows representing each sample and columns representing individual genes ( D ). Expression (TPM) of pyruvate dehydrogenase alpha 1 (PDHA1) and aconitase 1 (ACO1) was plotted ( E ). PDHA1 is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA; ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle. Significant differences are indicated by: * p < 0.05, ** p < 0.01, and *** p < 0.001. provides the semi-raw data from the RNASeq analysis that was used to generate the figures.

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: Overexpression of genes involved in oxidative phosphorylation in GL261 LDH-A KD cells. Transcripts per million (TPM) expression values were plotted in individual cell lines for genes directly involved in lactate metabolism and export (LDH-A, LDH-B, and SLC16A3) ( A ). Gene Set Enrichment Analysis (GSEA) for a single pathway (GO_OXIDATIVE_ PHOSPHORYLATION) is shown for each GBM cell line. The analysis shows the enrichment of this pathway in LDH-A-depleted vs. control cells ( B ). TPM values for enzymes involved in oxidative phosphorylation ( C ). The Z-transformed scores of individual genes within the oxidative phosphorylation (GO) pathway were plotted across each cell line (GL261, CT2A and ALTS1C1). The experiment was performed in triplicate, with rows representing each sample and columns representing individual genes ( D ). Expression (TPM) of pyruvate dehydrogenase alpha 1 (PDHA1) and aconitase 1 (ACO1) was plotted ( E ). PDHA1 is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA; ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle. Significant differences are indicated by: * p < 0.05, ** p < 0.01, and *** p < 0.001. provides the semi-raw data from the RNASeq analysis that was used to generate the figures.

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Over Expression, Phospho-proteomics, Expressing, Control, Transformation Assay

    The effect of LDH-A knock-down on cells in vitro and s.c. tumor growth, in vivo in immune-competent mice . Growth profiles and doubling times of GL261, ALTS1C1 and CT2A cells in vitro (Panels A – D ) (mean ± SEM) and tumors in C57BL/6 mice (Panels E – H ) with and without LDH-A shRNA knock-down (mean, ± SD). Note that the doubling times for GL261 NC tumors (Panel E) were estimated after the initial delay in tumor growth (0~40 days).

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: The effect of LDH-A knock-down on cells in vitro and s.c. tumor growth, in vivo in immune-competent mice . Growth profiles and doubling times of GL261, ALTS1C1 and CT2A cells in vitro (Panels A – D ) (mean ± SEM) and tumors in C57BL/6 mice (Panels E – H ) with and without LDH-A shRNA knock-down (mean, ± SD). Note that the doubling times for GL261 NC tumors (Panel E) were estimated after the initial delay in tumor growth (0~40 days).

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Knockdown, In Vitro, In Vivo, shRNA

    The effect of LDH-A knock-down on s.c. tumor growth in nude mice . Growth profiles of GL261, ALTS1C1 and CT2A tumors (Panels A – C ), and estimated tumor doubling times (Panel D ). Mean, ± SD. Comparison between tumor doubling times in nude mice and C57BL/6 mice (Panel E ).

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: The effect of LDH-A knock-down on s.c. tumor growth in nude mice . Growth profiles of GL261, ALTS1C1 and CT2A tumors (Panels A – C ), and estimated tumor doubling times (Panel D ). Mean, ± SD. Comparison between tumor doubling times in nude mice and C57BL/6 mice (Panel E ).

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Knockdown, Comparison

    Native-polyacrylamide gel electrophoresis LDH zymograms for ex vivo tissue and s.c. GL261, CT2A and ALTS1C2 tumors. Electrophoretic patterns in the heart and skeletal muscle as well as s.c. tumors from NC and LDH-A KD GL261, CT2A and ALTS1C1 tumors (Panel A ), corresponding LDH isoform profiles (Panel B ); n = five independent studies.

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: Native-polyacrylamide gel electrophoresis LDH zymograms for ex vivo tissue and s.c. GL261, CT2A and ALTS1C2 tumors. Electrophoretic patterns in the heart and skeletal muscle as well as s.c. tumors from NC and LDH-A KD GL261, CT2A and ALTS1C1 tumors (Panel A ), corresponding LDH isoform profiles (Panel B ); n = five independent studies.

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Polyacrylamide Gel Electrophoresis, Ex Vivo

    H&E and IHC staining for LDH-A and LDH-B protein expression in s.c. GL261 and CT2A tumors—both LDH-A KD and NC controls . H&E staining for GL261 NC and LDH-A KD tumors ( Aa ); LDH-A staining ( Ab ) and LDH-B staining ( Ac ). GL261 NC and LDH-A KD tumors were grown in immunocompromised nude mice. Quantification of percentage LDH-A tumor expression ( B ) and percentage LDH-B tumor expression ( C ); ±SEM. A similar presentation is shown for CT2A tumors, grown in immune-competent C57BL/6 mice (Panels D – F ).

    Journal: Cancers

    Article Title: LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth Responses

    doi: 10.3390/cancers14092303

    Figure Lengend Snippet: H&E and IHC staining for LDH-A and LDH-B protein expression in s.c. GL261 and CT2A tumors—both LDH-A KD and NC controls . H&E staining for GL261 NC and LDH-A KD tumors ( Aa ); LDH-A staining ( Ab ) and LDH-B staining ( Ac ). GL261 NC and LDH-A KD tumors were grown in immunocompromised nude mice. Quantification of percentage LDH-A tumor expression ( B ) and percentage LDH-B tumor expression ( C ); ±SEM. A similar presentation is shown for CT2A tumors, grown in immune-competent C57BL/6 mice (Panels D – F ).

    Article Snippet: The small 5-day tumors from GL261 NC and KD were stained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako).

    Techniques: Immunohistochemistry, Expressing, Staining